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On Friday it was confirmed that the Giallorosse will play the home leg of their UEFA Women's Champions League quarter-final tie at the historic stadium, which can host over 60,000 fans.
Club captain Elisa Bartoli, Rome-born and a Roma fan, made the announcement on social media.
The match will take place in March, with Alessandro Spugna's squad due to find out their opposition during February's draw.
Arsenal, Chelsea and Barcelona are the potential opposition.
Making their debut in Europe's top club competition for women, Roma have gone on to make the last-eight - beating the likes of Paris FC and Sparta Prague along the way.
Playing their home group stage matches in Latina, the Giallorosse ultimately finished second in their group behind perennial European heavyweights Wolfsburg, claiming 13 points from a possible 18.
The club was only formed in 2018, but has since gone on to lift the Coppa Italia in 2021 and the Supercoppa last year. Spugna's side currently sit top of the Serie A standings, having finished second last term.
Ticket information for the match will be announced in due course.
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