First-half goals from Moeka Minami and Valentina Giacinti ensured a 2-0 victory in front of a sold-out home crowd.
The Giallorosse made a number of changes to the starting line-up after last week's opening day win against Pomigliano, with Giada Greggi, Lucia Di Guiglielmo, and Elisa Bartoli making their first league starts.
Minami reacted quickest in the box to open the scoring, followed by Giacinti's cool finish into the bottom corner just before half-time.
In the second-half, AC Milan struggled to get into the game as Roma controlled possession throughout.
See more photos from the game in the gallery below!
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