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Di Francesco can call upon both Alessandro Florenzi and Bruno Peres, who were unavailable for the club's last match - against Inter Milan at the end of August.
The one major absentee is Patrik Schick, who suffered a minor problem in training on Friday, while left-back Rick Karsdorp has not been called up for this match as he continues his recovery after knee surgery.
Tuesday's game at the Stadio Olimpico gets underway at 20:45 CEST.
See the full squad list below.
AS Roma squad to face Atletico Madrid:
Alisson Becker
Andrea Romagnoli
Lukasz Skorupski
Juan Jesus
Aleksandar Kolarov
Hector Moreno
Federico Fazio
Bruno Peres
Kostas Manolas
Radja Nainggolan
Kevin Strootman
Lorenzo Pellegrini
Daniele De Rossi
Maxime Gonalons
Alessandro Florenzi
Gerson
Diego Perotti
Edin Dzeko
Cengiz Under
Gregoire Defrel
Stephan El Shaarawy
Imperium Partners
Centurion Partners
